https://pubmed.ncbi.nlm.nih.gov/32990953/ Biotechnol Bioeng. 2021 Jan;118(1):442-452. doi: 10.1002/bit.27582. Epub 2020 Oct 13. B N Napiwocki 1 2, D Lang 3, A Stempien 1 2, J Zhang 3, R Vaidyanathan 3, J C Makielski 3, L L Eckhardt 3, A V Glukhov 3, T J Kamp 3 4, W C Crone 1 2 5 PMID: 32990953 PMCID: PMC8214444 DOI: 10.1002/bit.27582 Abstract Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have emerged as an exciting new tool for cardiac research and can serve as a preclinical platform …

Aligned human cardiac syncytium for in vitro analysis of electrical, structural, and mechanical readouts 더 보기 »

https://www.mdpi.com/2073-4409/11/6/951/htm?msclkid=2320efa7a69611ecbfb2e3ff4e60dc12 by Jyotsna Joshi 1,†,Bing Xu 1,2,†,Michael Rubart 3,Yun Chang 4,Xiaoping Bao 4,Hari P. Chaliki 1,Luis R. Scott 1 andWuqiang Zhu 1,5,6,* Cells2022, 11(6), 951; https://doi.org/10.3390/cells11060951Received: 24 February 2022 / Revised: 7 March 2022 / Accepted: 8 March 2022 / Published: 10 March 2022 Abstract Background: Cellular transplantation has emerged as promising approach for treating cardiac diseases. However, a poor engraftment rate limits our understanding on how transplanted cardiomyocytes contribute to cardiac function …

Optogenetic Control of Engrafted Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Live Mice: A Proof-of-Concept Study 더 보기 »

https://physoc.onlinelibrary.wiley.com/doi/10.14814/phy2.15045?msclkid=5cdb6f0ea69911ec94ea077be24f81c1 Physiological ReportsVolume 9, Issue 19 e15045 B.N. Napiwocki,A. Stempien,D. Lang,R.A. Kruepke,G. Kim,J. Zhang,L.L. Eckhardt,A.V. Glukhov,T.J. Kamp,W.C. CroneFirst published: 07 October 2021https://doi.org/10.14814/phy2.15045 Abstract In native heart tissue, cardiac fibroblasts provide the structural framework of extracellular matrix (ECM) while also influencing the electrical and mechanical properties of cardiomyocytes. Recent advances in the field of stem cell …

Micropattern platform promotes extracellular matrix remodeling by human PSC-derived cardiac fibroblasts and enhances contractility of co-cultured cardiomyocytes 더 보기 »

https://pubmed.ncbi.nlm.nih.gov/33628268/ Stem Cells Int. 2021 Feb 6;2021:6657597. doi: 10.1155/2021/6657597. eCollection 2021. Fuquan Chen 1, Miao Zhang 1, Xiao Feng 1, Xiaomin Li 1, Haotian Sun 1, Xinyi Lu 1 PMID: 33628268 PMCID: PMC7884122 DOI: 10.1155/2021/6657597 Abstract Pluripotency and self-renewal of embryonic stem cells (ESCs) are marked by core transcription regulators such as Oct4, Sox2, and Nanog. Another important marker of pluripotency is the long noncoding RNA (lncRNA). Here, we …

Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency 더 보기 »

https://pubmed.ncbi.nlm.nih.gov/31523707/ Sci Adv. 2019 Sep 6;5(9):eaaw2459. doi: 10.1126/sciadv.aaw2459. eCollection 2019 Sep. Mark A Skylar-Scott 1 2, Sebastien G M Uzel 1 2, Lucy L Nam 1 2, John H Ahrens 1 2, Ryan L Truby 1 2, Sarita Damaraju 1 2, Jennifer A Lewis 1 2 PMID: 31523707 PMCID: PMC6731072 DOI: 10.1126/sciadv.aaw2459 AbstractEngineering organ-specific tissues for therapeutic applications is a grand challenge, requiring the fabrication and maintenance of densely cellular constructs composed of ~108 cells/ml. Organ building blocks …

Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels 더 보기 »

https://pubmed.ncbi.nlm.nih.gov/31031139/ Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25. Hana Benchetrit 1, Mohammad Jaber 1, Valery Zayat 1, Shulamit Sebban 1, Avital Pushett 1, Kirill Makedonski 1, Zvi Zakheim 1, Ahmed Radwan 1, Noam Maoz 1, Rachel Lasry 1, Noa Renous 1, Michal Inbar 1, Oren Ram 2, Tommy Kaplan 3, Yosef Buganim 4 PMID: 31031139 PMCID: PMC6561721 DOI: 10.1016/j.stem.2019.03.018 Abstract Following fertilization, totipotent cells undergo asymmetric cell divisions, resulting in three distinct cell types in the late pre-implantation blastocyst: epiblast (Epi), primitive …

Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts 더 보기 »