https://pubmed.ncbi.nlm.nih.gov/35144661/ Paulo C M Urbano 1 2, Hamish C K Angus 1, Safina Gadeock 3, Michael Schultz 2 4, Roslyn A Kemp 5Affiliations expand PMID: 35144661 PMCID: PMC8830126 DOI: 10.1186/s13104-022-05925-4 Abtract Objective: Human intestinal organoids (hIOs) have potential as a model for investigating intestinal diseases. The hIO system faces logistic challenges including limited access to biopsies or low expression of epithelial cell types. Previous research identified the feasibility of tissue from …

Assessment of source material for human intestinal organoid culture for research and clinical use 더 보기 »

https://pubmed.ncbi.nlm.nih.gov/33451982/ Cancer Discov. 2021 Jun;11(6):1562-1581. doi: 10.1158/2159-8290.CD-20-1109. Epub 2021 Jan 15. Yuan-Hung Lo 1, Kevin S Kolahi 2, Yuhong Du 3, Chiung-Ying Chang 2, Andrey Krokhotin 4, Ajay Nair 5, Walter D Sobba 1, Kasper Karlsson 1 6, Sunny J Jones 5, Teri A Longacre 2, Amanda T Mah 1, Bahar Tercan 7, Alexandra Sockell 6, Hang Xu 6, Jose A Seoane 6, Jin Chen 8 9, Ilya Shmulevich 7, Jonathan S Weissman 8, Christina Curtis 6, Andrea Califano 5, Haian Fu 3, Gerald R Crabtree 2 4 10, Calvin J Kuo 11Affiliations expand PMID: 33451982 PMCID: PMC8346515 DOI: 10.1158/2159-8290.CD-20-1109 Abstract Mutations in ARID1A rank among the most common molecular …

A CRISPR/Cas9-Engineered ARID1A-Deficient Human Gastric Cancer Organoid Model Reveals Essential and Nonessential Modes of Oncogenic Transformation 더 보기 »

https://pubmed.ncbi.nlm.nih.gov/33207077/ Mol Oncol. 2021 Feb;15(2):679-696. doi: 10.1002/1878-0261.12857. Epub 2020 Dec 5. Jeong-Yun Choi 1, Haeseung Lee 2, Eun-Ji Kwon 1, Hyeon-Joon Kong 1, Ok-Seon Kwon 3, Hyuk-Jin Cha 1 4Affiliations expand PMID: 33207077 PMCID: PMC7858114 DOI: 10.1002/1878-0261.12857 Abstract The acquisition of chemoresistance remains a major cause of cancer mortality due to the limited accessibility of targeted or immune therapies. However, given that severe alterations of molecular features during epithelial-to-mesenchymal transition (EMT) …

TGFβ promotes YAP-dependent AXL induction in mesenchymal-type lung cancer cells 더 보기 »

https://pubmed.ncbi.nlm.nih.gov/33732801/ Bio Protoc. 2021 Feb 5;11(3):e3914. doi: 10.21769/BioProtoc.3914. Ryutaro Kajihara 1 2 3, Tadahiro Numakawa 1, Takumi Era 1Affiliations expand PMID: 33732801 PMCID: PMC7952933 DOI: 10.21769/BioProtoc.3914 Abstract Human induced pluripotent stem cells (iPSCs) and their progeny displaying tissue-specific characteristics have paved the way for regenerative medicine and research in various fields such as the elucidation of the pathological mechanism of diseases and the discovery of drug …

Rapid and Simplified Induction of Neural Stem/Progenitor Cells (NSCs/NPCs) and Neurons from Human Induced Pluripotent Stem Cells (hiPSCs) 더 보기 »

https://pubmed.ncbi.nlm.nih.gov/33141462/ J Neurosci Res. 2021 Jan;99(1):110-123. doi: 10.1002/jnr.24747. Epub 2020 Nov 3. Lucie Y Ahn 1 2, Giuliana C Coatti 1, Jingyi Liu 3, Evren Gumus 4 5, Ashleigh E Schaffer 1 6, Helen C Miranda 1 7Affiliations expand PMID: 33141462 PMCID: PMC7756336 DOI: 10.1002/jnr.24747 Abstract ACTL6B is a component of the neuronal BRG1/brm-associated factor (nBAF) complex, which is required for chromatin remodeling in postmitotic neurons. We recently reported biallelic pathogenic variants in ACTL6B …

An epilepsy-associated ACTL6B variant captures neuronal hyperexcitability in a human induced pluripotent stem cell model 더 보기 »

https://pubmed.ncbi.nlm.nih.gov/30183752/ PLoS One. 2018 Sep 5;13(9):e0203126. doi: 10.1371/journal.pone.0203126. eCollection 2018. Sheryl M Southard 1, Rama P Kotipatruni 1, William L Rust 1 PMID: 30183752 PMCID: PMC6124757 DOI: 10.1371/journal.pone.0203126 Abstract Induced pluripotent stem cell (iPSC) technology enables the creation and selection of pluripotent cells with specific genetic traits. This report describes a pluripotent cell line created specifically to form replacement pancreatic cells as a …

Generation and selection of pluripotent stem cells for robust differentiation to insulin-secreting cells capable of reversing diabetes in rodents 더 보기 »