#Anti-Human CD3 Purified ; #05131-20 ;
J Clin Endocrinol Metabc. 2024 Apr 9:dgae212.
Daniel Marrero-Rodriguez 1, Victor A Cortes-Morales 1 2, Amayrani Cano-Zaragoza 1, Florencia Martinez-Mendoza 1, Jacobo Kerbel-Suton 1, Sandra Vela-Patiño 1, Alejandra Chavez-Santoscoy 3, Silvia Hinojosa-Alvarez 3, Jesus Hernandez-Perez 3, Erick Gomez-Apo 4, Guadalupe R Fajardo-Orduña 2, Keiko Taniguchi-Ponciano 1, Juan Jose Montesinos 2, Moises Mercado 1
Affiliations expand
- PMID: 38589986
- DOI: 10.1210/clinem/dgae212
Abstract
Background: The tumor microenvironment (TME) includes diverse cellular components such as mesenchymal stem cells (MSC) and immune cells among others. MSC have been isolated from different tumors and they favor tumor cell growth, however, their role in pituitary tumors (PT) remains unknown. Herein we report the presence of MSCs in 2 ACTH-secreting PT causing Cushing disease (MCU), 2 nonfunctioning adenomas of gonadotrope differentiation (MNF) and 2 non tumoral pituitary glands (MS).
Methods: We have analyzed their transcriptomic profiles by RNAseq and compared MSC in terms of their immunosuppressive effects against lymphoid T cell and macrophage populations by means of co-cultures and flow cytometry.
Results: Our transcriptomic analysis revealed molecular differences between MSC derived from non-tumoral pituitaries and MSC derived from PT. Two distinct subpopulations of MSC, one displaying immunosuppressive properties and the other with increased pro-proliferative capabilities, regardless of their origin. MSC derived from ACTH- and nonfunctioning PT, but not those derived from non-tumoral glands significantly inhibited the proliferation of activated T cells, favored the generation of Tregs and promote M2 macrophage polarization. Such immunosuppressive effects were correlated with an upregulation of programmed death ligand 1 and intracellular expression of macrophage colony stimulating factor (M-CSF) and IL-10. Importantly, MSC derived from ACTH-PT showed a higher immunosuppressive potential than MSC isolated from nonfunctioning tumors.
Conclusion: This study demonstrates the presence of at least two MSC subpopulations in the pituitary gland and suggests that immunosuppressive effects of MSC may have important implications in PT growth.