Recombinant laminin-511 (BG iMatrix-511)
BG iMatrix 511 Silk 는 CHO-S cells에서 생산된 laminin-511 E8 단편으로, animal-free, and xeno-free 입니다.
Laminin-511은 세포 표면에 위치한 integrin α6ß1에 결합하는 것으로 잘 알려져 있으며, ESC 및 iPSC를 포함한 다양한 세포 유형의 줄기세포 배양 배지의 기질로 작용합니다.
재조합 laminin-511 E8 단편은 알파, 베타 및 감마 사슬의 C-말단 영역으로 구성된, laminin의 절단된 기능적 형태입니다.
Laminin-511 E8(150 kDa)은 alpha 6 beta 1 integrin에 결합할 수 있는 완전한 기능을 유지합니다.
350 ug 사이즈는 2 x 175 ug 바이알로 공급됩니다. 1050 ug 사이즈는 6 x 175 ug 바이알로 제공됩니다.
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[ 최신 제품 인용 논문 ]
- Hsueh YC, Pratt RE, Dzau VJ, Hodgkinson CP. Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2. Sci Rep. 2023 Mar 9;13(1):3920. doi: 10.1038/s41598-023-31144-3. PMID: 36894665; PMCID: PMC9998650.
- Balafkan N, Mostafavi S, Schubert M, Siller R, Liang KX, Sullivan G, Bindoff LA. A method for differentiating human induced pluripotent stem cells toward functional cardiomyocytes in 96-well microplates. Sci Rep. 2020 Oct 28;10(1):18498. doi: 10.1038/s41598-020-73656-2. PMID: 33116175; PMCID: PMC7595118.
ADDITIONAL INFORMATION
Applications:
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Cell Culture
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Research Interest: | Stem Cell Research |
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Cell Type: | Stem Cells |
Application: | Cell Culture |
Application Notes:
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Formulation:
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0.5mg/mL in PBS(-).
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References:
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Taniguchi, Y., Ido, H., Sanzen, N., Hayashi, M., Sato-Nishiuchi, R., Futaki, S., & Sekiguchi, K. . The C-terminal region of laminin β chains modulates the integrin binding affinities of laminins. Journal of Biological Chemistry, 284(12), 7820-7831, (2009) Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, Y., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K., & Kawase, E. . Laminin E8 fragment support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nature communications, 3(1236), 1-10, (2012) Burridge, P. W., Matsa, E., Shukla, P., Lin, Z. C., Churko, J. M., Ebert, A. D., Lan, F., Diecke, S., Huber, B., Mordwinkin, N. M., Plews, J. R., Abilez, O. J., Cui, B., Gold, J. D., & Wu, J. C. . Chemically defined generation of human cardiomyocytes. Nature methods, 11(8), 855-860, (2014) Nakagawa, M., Taniguchi, Y., Senda, S., Takizawa, N., Ichisaka, T., Asano, K., Morizane, A., Doi, D., Takahashi, J., Nishizawa, M., Yoshida, Y., Toyoda, T., Osafune, K., Sekiguchi, K., & Yamanaka, S. . A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Scientific reports, 4(3594), 1-7, (2014) Takayama, K., Mitani, S., Nagamoto, Y., Sakurai, F., Tachibana, M., Taniguchi, Y., Sekiguchi, K., Mizuguchi, H.. Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells. Biochemical and biophysical research communications, 474(1), 91-96, (2016) Musah, S., Dimitrakakis, N., Camacho, D. M., Church, G. M., Ingber, D. E.. Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a lomerulus Chip. Nature protocols, 13(7), 1662, (2018) |
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