Viability Dye 506
Viability Dye 506은 flow cytometry 분석시 죽은 세포를 제외하는 데 사용됩니다.
Viability Dye 506은 세포 표면의 아민기(amine) 및 세포내의 아민기에 비가역적으로 결합합니다.
Viability Dye 506은 원형질막의 완결성을 상실한 세포자멸사 후기의 세포 또는 죽은 세포들의 더 높은 농도의 아민과 공유 결합하여 훨씬 더 강력한 형광 신호를 생성합니다. Viability Dye 506의 가장 큰 장점은 propidium iodide 및 7-AAD에 비해 세포 세척, 고정, 투과화 및 세포내 염색 동안 형광이 소실되지 않는다는 것입니다.
Viability Dye 506은 보라색(405nm) 레이저로 excitation 될 수 있으며 510/550 대역 통과 필터로도 감지할 수 있는 506nm의 emissino peak을 가집니다.
(500 test kit는 5 tubes x 100 test 로 제공됩니다.)
[제품인용최신논문]
- Song Y, Zhang X, Desmarais JA, Nagano M. Postnatal development of mouse spermatogonial stem cells as determined by immunophenotype, regenerative capacity, and long-term culture-initiating ability: a model for practical applications. Sci Rep. 2024 Jan 27;14(1):2299. doi: 10.1038/s41598-024-52824-8. PMID: 38280889; PMCID: PMC10821885.
- Lee EJ, Lee KJ, Jung S, Park KH, Park SI. Mobilization of monocytic myeloid-derived suppressor cells is regulated by PTH1R activation in bone marrow stromal cells. Bone Res. 2023 Apr 21;11(1):22. doi: 10.1038/s41413-023-00255-y. PMID: 37085481; PMCID: PMC10121701.
- Zhang Y, Wang W, Min J, Liu S, Wang Q, Wang Y, Xiao Y, Li X, Zhou Z, Liu S. ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression. Cell Rep. 2023 Jun 19;42(6):112654. doi: 10.1016/j.celrep.2023.112654. PMID: 37342906.
- Zheng W, D’Aiuto L, Demers MJ, Muralidaran V, Wood JA, Wesesky M, Chattopadhyay A, Nimgaonkar VL. Insights into bioinformatic approaches for repurposing compounds as anti-viral drugs. Antivir Chem Chemother. 2021 Jan-Dec;29:20402066211036822. doi: 10.1177/20402066211036822. PMID: 34463534; PMCID: PMC8411619.
- Zheng W, Klammer AM, Naciri JN, Yeung J, Demers M, Milosevic J, Kinchington PR, Bloom DC, Nimgaonkar VL, D’Aiuto L. Patterns of Herpes Simplex Virus 1 Infection in Neural Progenitor Cells. J Virol. 2020 Jul 30;94(16):e00994-20. doi: 10.1128/JVI.00994-20. Erratum in: J Virol. 2021 Jan 28;95(4): PMID: 32493817; PMCID: PMC7394888.
- D’Aiuto L, McNulty J, Hartline C, Demers M, Kalkeri R, Wood J, McClain L, Chattopadhyay A, Zhi Y, Naciri J, Smith A, Yolken R, Chowdari K, Zepeda-Velazquez C, Dokuburra CB, Marques E, Ptak R, Kinchington P, Watkins S, Prichard M, Bloom D, Nimgaonkar V. R430: A potent inhibitor of DNA and RNA viruses. Sci Rep. 2018 Nov 9;8(1):16662. doi: 10.1038/s41598-018-33904-y. PMID: 30413769; PMCID: PMC6226428.
Catalog Number : 62210-00
ADDITIONAL INFORMATION
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Applications:
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FC
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| Application: | FC |
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Application Notes:
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The antibody has been analyzed for quality through the flow cytometric analysis of the relevant cell type. The antibody can be used at less than or equal to 5 μL per test. A test is the amount of antibody required to stain a cell sample in the final volume of 100 μL.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2. |
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References:
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Perfetto, S. P., Chattopadhyay, P. K., Lamoreaux, L., Nguyen, R., Ambrozak, D., Koup, R. A., & Roederer, M. (2006). Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry. Journal of immunological methods, 313(1), 199-208
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